Expressing:Article Title: ACY1 regulating PTEN/PI3K/AKT signaling in the promotion of non-small cell lung cancer progression
Article Snippet: Target bands of proteins were visualized with an efficient chemiluminescence (ECL) western blotting kit (Millipore, Boston, MA, USA). .. Relative expression of proteins was assessed with the 2 −∆∆CT method. table ft1 table-wrap mode="anchored" t5 caption a7 Names Dilution Item No. Company ACY1 1:500 AF2900 R&D Systems, MN, USA PI3K 1:500 4252 Cell Signaling Technology, Beverly, CA, USA p-PI3K 1:500 4228 Cell Signaling Technology, Beverly, CA, USA AKT 1:500 9272 Cell Signaling Technology, Beverly, CA, USA p-AKT 1:500 13038 Cell Signaling Technology, Beverly, CA, USA mTOR 1:500 ab2732 Abcam, Cambridge, MA, USA PTEN 1:500 9552 Cell Signaling Technology, Beverly, CA, USA Ub 1:500 3933 Cell Signaling Technology, Beverly, CA, USA GAPDH 1:500 TA-08 Zhongshanjinqiao, Beijing, China Open in a separate window ACY1, aminoacylase 1; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. caption a8 Antibodies Immunoprecipitation assay NSCLC cells were treated with sh-NC, sh-ACY1, OE-NC, or OE-ACY1 for 48 hours before being rinsed with cold phosphate-buffered saline (PBS) and lysed in immunoprecipitation (IP) lysis buffer (Thermo Fisher Scientific). ..
Article Title: ACY1 regulating PTEN/PI3K/AKT signaling in the promotion of non-small cell lung cancer progression
Article Snippet: Target bands of proteins were visualized with an efficient chemiluminescence (ECL) western blotting kit (Millipore, Boston, MA, USA). .. Relative expression of proteins was assessed with the 2 −∆∆CT method. table ft1 table-wrap mode="anchored" t5 caption a7 Names Dilution Item No. Company ACY1 1:500 AF2900 R&D Systems, MN, USA PI3K 1:500 4252 Cell Signaling Technology, Beverly, CA, USA p-PI3K 1:500 4228 Cell Signaling Technology, Beverly, CA, USA AKT 1:500 9272 Cell Signaling Technology, Beverly, CA, USA p-AKT 1:500 13038 Cell Signaling Technology, Beverly, CA, USA mTOR 1:500 ab2732 Abcam, Cambridge, MA, USA PTEN 1:500 9552 Cell Signaling Technology, Beverly, CA, USA Ub 1:500 3933 Cell Signaling Technology, Beverly, CA, USA GAPDH 1:500 TA-08 Zhongshanjinqiao, Beijing, China Open in a separate window ACY1, aminoacylase 1; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. caption a8 Antibodies .. NSCLC cells were treated with sh-NC, sh-ACY1, OE-NC, or OE-ACY1 for 48 hours before being rinsed with cold phosphate-buffered saline (PBS) and lysed in immunoprecipitation (IP) lysis buffer (Thermo Fisher Scientific).
Immunoprecipitation:Article Title: ACY1 regulating PTEN/PI3K/AKT signaling in the promotion of non-small cell lung cancer progression
Article Snippet: Target bands of proteins were visualized with an efficient chemiluminescence (ECL) western blotting kit (Millipore, Boston, MA, USA). .. Relative expression of proteins was assessed with the 2 −∆∆CT method. table ft1 table-wrap mode="anchored" t5 caption a7 Names Dilution Item No. Company ACY1 1:500 AF2900 R&D Systems, MN, USA PI3K 1:500 4252 Cell Signaling Technology, Beverly, CA, USA p-PI3K 1:500 4228 Cell Signaling Technology, Beverly, CA, USA AKT 1:500 9272 Cell Signaling Technology, Beverly, CA, USA p-AKT 1:500 13038 Cell Signaling Technology, Beverly, CA, USA mTOR 1:500 ab2732 Abcam, Cambridge, MA, USA PTEN 1:500 9552 Cell Signaling Technology, Beverly, CA, USA Ub 1:500 3933 Cell Signaling Technology, Beverly, CA, USA GAPDH 1:500 TA-08 Zhongshanjinqiao, Beijing, China Open in a separate window ACY1, aminoacylase 1; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. caption a8 Antibodies Immunoprecipitation assay NSCLC cells were treated with sh-NC, sh-ACY1, OE-NC, or OE-ACY1 for 48 hours before being rinsed with cold phosphate-buffered saline (PBS) and lysed in immunoprecipitation (IP) lysis buffer (Thermo Fisher Scientific). ..
Saline:Article Title: ACY1 regulating PTEN/PI3K/AKT signaling in the promotion of non-small cell lung cancer progression
Article Snippet: Target bands of proteins were visualized with an efficient chemiluminescence (ECL) western blotting kit (Millipore, Boston, MA, USA). .. Relative expression of proteins was assessed with the 2 −∆∆CT method. table ft1 table-wrap mode="anchored" t5 caption a7 Names Dilution Item No. Company ACY1 1:500 AF2900 R&D Systems, MN, USA PI3K 1:500 4252 Cell Signaling Technology, Beverly, CA, USA p-PI3K 1:500 4228 Cell Signaling Technology, Beverly, CA, USA AKT 1:500 9272 Cell Signaling Technology, Beverly, CA, USA p-AKT 1:500 13038 Cell Signaling Technology, Beverly, CA, USA mTOR 1:500 ab2732 Abcam, Cambridge, MA, USA PTEN 1:500 9552 Cell Signaling Technology, Beverly, CA, USA Ub 1:500 3933 Cell Signaling Technology, Beverly, CA, USA GAPDH 1:500 TA-08 Zhongshanjinqiao, Beijing, China Open in a separate window ACY1, aminoacylase 1; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. caption a8 Antibodies Immunoprecipitation assay NSCLC cells were treated with sh-NC, sh-ACY1, OE-NC, or OE-ACY1 for 48 hours before being rinsed with cold phosphate-buffered saline (PBS) and lysed in immunoprecipitation (IP) lysis buffer (Thermo Fisher Scientific). ..
Lysis:Article Title: ACY1 regulating PTEN/PI3K/AKT signaling in the promotion of non-small cell lung cancer progression
Article Snippet: Target bands of proteins were visualized with an efficient chemiluminescence (ECL) western blotting kit (Millipore, Boston, MA, USA). .. Relative expression of proteins was assessed with the 2 −∆∆CT method. table ft1 table-wrap mode="anchored" t5 caption a7 Names Dilution Item No. Company ACY1 1:500 AF2900 R&D Systems, MN, USA PI3K 1:500 4252 Cell Signaling Technology, Beverly, CA, USA p-PI3K 1:500 4228 Cell Signaling Technology, Beverly, CA, USA AKT 1:500 9272 Cell Signaling Technology, Beverly, CA, USA p-AKT 1:500 13038 Cell Signaling Technology, Beverly, CA, USA mTOR 1:500 ab2732 Abcam, Cambridge, MA, USA PTEN 1:500 9552 Cell Signaling Technology, Beverly, CA, USA Ub 1:500 3933 Cell Signaling Technology, Beverly, CA, USA GAPDH 1:500 TA-08 Zhongshanjinqiao, Beijing, China Open in a separate window ACY1, aminoacylase 1; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. caption a8 Antibodies Immunoprecipitation assay NSCLC cells were treated with sh-NC, sh-ACY1, OE-NC, or OE-ACY1 for 48 hours before being rinsed with cold phosphate-buffered saline (PBS) and lysed in immunoprecipitation (IP) lysis buffer (Thermo Fisher Scientific). ..
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